Eye tracking is a powerful method of studying behaviour and brain function. It not only provides direct measurements of eye movements, blinks, and gaze position, but information about pupil size has been used as an indirect index of attention and arousal. This workshop will provide a brief overview of the physiological basis, signal types, and analysis methods of eye tracking data. In the practical part of the workshop, participants will learn how to set up the eye tracker and measure participants’ responses in several example experiments.